mobitec的巨大芽孢杆菌表达系统
hp-Vector表达系统megaterium芽孢杆菌
hp-Vector expression Systems for Bacillus megaterium
Features:
High performance vectors with optimized sequence
Protein yield up to 10 times enhanced compared to protein expression with basic plasmid
Plasmids with established MCS
Encoding C- or N-terminal His-tag for versatile purification (native, 6xHis-tag)
Secretion with LipA or YocH signal peptide up to 9 fold increased
optimized high performance (hp) vectors for Bacillus megaterium!
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable to most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence and a 6xHis-tag.
With our new hp-vectors yields up to 10-fold higher compared to the basic plasmids are possible. All plasmids have established multiple cloning sites (MCS) for versatile cloning. Furthermore we offer vectors encoding C- or N-terminal His-tags for easy purification. The protein secretion with LipA or YocH signal peptides is up to nine-fold increased. Induction of protein expression of all vectors is achieved by the tightly regulated and efficiently inducible xylose operon.
Versatile system with a wide range of vectors
The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale.
B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.
B. megaterium protoplasts specifically optimized for transformation
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.
For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.
Order Information, shipping and storage
Please click on the respective order # for further details.
order# description amount
BMEG02 Bacillus megaterium protoplasts ready for transformation (strain WH320;1)
Material is sufficient for 4 transformations plus control experiment. 5 x 500 µl
BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 µl
BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg
BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg
BMEG30 p3STOP1623hp, shuttle vector, lyophilized DNA 10 μg
BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg
BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 µg
shipped at RT, protoplasts shipped on dry ice
store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C
All vectors are E. coli / B. megaterium shuttle vectors
Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase and use of protoplasts must be thoroughly planned.
